Hotspot

trnV-GAC-rps12

IRa · 107266-108541 · 1276 bp

Back to Helixanthera

Pi 0.0223

MarkerSeek score 73.00

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1276
variable_sites: 28
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1372-1593 bp	1.0	79.2
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1376-1597 bp	1.0	79.2
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.8	57.7	1371-1592 bp	1.0	79.2
trnV-GAC-rps12_p4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.3	57.7	1374-1595 bp	1.0	78.2
trnV-GAC-rps12_p5	GATGACTTCCACCACGTCAA	ACGTAAGGCAGAGTTTGGTT	50.0	45.0	57.8	57.4	1362-1583 bp	1.0	76.2