Hotspot

trnL-UAG-ccsA

SSC · 93045-93245 · 201 bp

Back to Helixanthera

Pi 0.041

MarkerSeek score 78.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 201
variable_sites: 8
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TTGGTAAACACGCTGCTCTT	TGTTCAAAAGTCGAAAACAGCA	45.0	36.4	58.0	57.6	289-377 bp	1.0	63.6
trnL-UAG-ccsA_p2	AACACGCTGCTCTTAGGAAG	TGTTCAAAAGTCGAAAACAGCA	50.0	36.4	57.9	57.6	283-371 bp	1.0	63.3
trnL-UAG-ccsA_p3	AAAGGCCGCTATGGTGAAAT	TGTTCAAAAGTCGAAAACAGCA	45.0	36.4	57.9	57.6	308-396 bp	1.0	62.6
trnL-UAG-ccsA_p4	AAGGCCGCTATGGTGAAATT	TGTTCAAAAGTCGAAAACAGCA	45.0	36.4	57.9	57.6	307-395 bp	1.0	62.6
trnL-UAG-ccsA_p5	ATTGGTAAACACGCTGCTCT	TGTTCAAAAGTCGAAAACAGCA	45.0	36.4	57.8	57.6	290-378 bp	1.0	62.5