Hotspot

trnP-UGG-psaJ

LSC · 54012-54350 · 339 bp

Back to Helixanthera

Pi 0.012

MarkerSeek score 62.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 4
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	GTACTTAGTACTGGTGCCGC	50.0	55.0	58.0	58.1	408-410 bp	1.0	81.4
trnP-UGG-psaJ_p2	TACCAAACTGCGCTACATCC	ACGCATCTGGGAAAAATCGA	50.0	45.0	58.0	57.9	470-472 bp	1.0	80.9
trnP-UGG-psaJ_p3	AAACAAACGCGCTACCAAAC	GTACTTAGTACTGGTGCCGC	45.0	55.0	58.2	58.1	420-422 bp	1.0	80.1
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	GTACTTAGTACTGGTGCCGC	45.0	55.0	58.2	58.1	422-424 bp	1.0	80.1
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	GTACTTAGTACTGGTGCCGC	45.0	55.0	57.8	58.1	429-431 bp	1.0	79.9