Hotspot

trnF-GAA-trnM-CAU

LSC · 40420-40963 · 544 bp

Back to Helixanthera

Pi 0.0227

MarkerSeek score 58.50

Primer available yes

Label trnF-GAA-trnM-CAU

Feature Metadata

label_name: trnF-GAA-trnM-CAU
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 12
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnM-CAU_p1	CCTCGTGTCACCAGTTCAAA	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.1	58.1	620-624 bp	1.0	82.7
trnF-GAA-trnM-CAU_p2	GGGGATATGGCGAAATTGGT	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.0	58.1	1278-1284 bp	1.0	82.0
trnF-GAA-trnM-CAU_p3	GGGGATATGGCGAAATTGGT	TTGAGAATGCGCCTTAACGA	50.0	45.0	58.0	57.9	1796-1808 bp	1.0	81.6
trnF-GAA-trnM-CAU_p4	GGGGATATGGCGAAATTGGT	AAGAAGCCCGTCAAACTCTG	50.0	50.0	58.0	58.1	1672-1684 bp	1.0	81.5
trnF-GAA-trnM-CAU_p5	GGGGATATGGCGAAATTGGT	CAAGAAGCCCGTCAAACTCT	50.0	50.0	58.0	58.1	1673-1685 bp	1.0	81.5