Hotspot

ycf3-trnS-GGA

LSC · 37093-37650 · 558 bp

Back to Helixanthera

Pi 0.0223

MarkerSeek score 73.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 12
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	GAATTGGGTGGGTGAAGTGT	47.6	50.0	58.0	58.0	667-686 bp	1.0	75.3
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	GTGAATTGGGTGGGTGAAGT	47.6	50.0	58.0	58.0	669-688 bp	1.0	75.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	CAGTGAATTGGGTGGGTGAA	47.6	50.0	58.0	58.0	671-690 bp	1.0	75.3
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	TGAATTGGGTGGGTGAAGTG	47.6	50.0	58.0	58.0	668-687 bp	1.0	75.3
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	628-647 bp	1.0	74.8