Hotspot

rps14-psaB

LSC · 29832-29976 · 145 bp

Back to Helixanthera

Pi 0.014

MarkerSeek score 61.90

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 2
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	ACTCAATGAGGGAACTTTGCT	TTGATTGCCTCCACATCAGG	42.9	50.0	57.5	57.9	290-292 bp	1.0	67.9
rps14-psaB_p2	CACTCAATGAGGGAACTTTGC	TTGATTGCCTCCACATCAGG	47.6	50.0	57.4	57.9	291-293 bp	1.0	67.5
rps14-psaB_p3	ACTCAATGAGGGAACTTTGCT	GCTCTTTCCATTGTGCAAGC	42.9	50.0	57.5	58.3	368-370 bp	1.0	66.4
rps14-psaB_p4	ACTCAATGAGGGAACTTTGCT	TGGCTCTTTCCATTGTGCAA	42.9	45.0	57.5	58.3	370-372 bp	1.0	66.4
rps14-psaB_p5	CACTCAATGAGGGAACTTTGC	GCTCTTTCCATTGTGCAAGC	47.6	50.0	57.4	58.3	369-371 bp	1.0	66.0