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trnC-GCA-petN

LSC · 22007-22218 · 212 bp

Back to Helixanthera

Pi 0.0099

MarkerSeek score 62.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 2
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAT	50.0	45.0	58.1	58.2	266-276 bp	1.0	81.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TAAAGCAGCCCAAGCGATAC	50.0	50.0	58.1	58.3	264-274 bp	1.0	80.2
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	ATTAAAGCAGCCCAAGCGAT	50.0	45.0	57.9	58.2	318-328 bp	1.0	80.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCGATA	50.0	45.0	58.1	57.6	265-275 bp	1.0	79.6
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	TAAAGCAGCCCAAGCGATAC	50.0	50.0	57.9	58.3	316-326 bp	1.0	79.2