Hotspot

atpH-atpI

LSC · 8833-9434 · 602 bp

Back to Helixanthera

Pi 0.00971

MarkerSeek score 66.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 5
pi: 0.00971
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	632-687 bp	1.0	82.7
atpH-atpI_p2	AACGGAAGCAGCAGAAATCA	CACTCGAGGGCCATCATTAG	45.0	55.0	58.1	57.8	597-652 bp	1.0	82.5
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	TCACTCGAGGGCCATCATTA	45.0	50.0	58.1	58.2	598-653 bp	1.0	82.1
atpH-atpI_p4	GAAGAAAGCCCTACAGCCAA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	664-719 bp	1.0	81.9
atpH-atpI_p5	GAAGAAAGCCCTACAGCCAA	CACTCGAGGGCCATCATTAG	50.0	55.0	57.8	57.8	629-684 bp	1.0	81.7