Hotspot

trnR-UCU-atpA

LSC · 5236-5454 · 219 bp

Back to Helixanthera

Pi 0.125

MarkerSeek score 75.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 21
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	ACGCTTTATATTTCAGGAAACA	47.6	31.8	57.3	54.1	229-275 bp	1.0	50.7
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AGAACGCTTTATATTTCAGGAAACA	47.6	32.0	57.3	57.3	232-278 bp	1.0	50.6
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GAACGCTTTATATTTCAGGAAACA	47.6	33.3	57.3	56.0	231-277 bp	1.0	50.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AGAACGCTTTATATTTCAGGAAACAA	47.6	30.8	57.3	57.9	232-278 bp	1.0	50.6
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	AGAACGCTTTATATTTCAGGAAACA	47.6	32.0	56.9	57.3	267-313 bp	1.0	49.8