Hotspot

ycf1

SSC · 124481-129982 · 5502 bp

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Pi 0.0255

MarkerSeek score 55.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5502
variable_sites: 207
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCAATAACGATTCATACAAGCT	AGAGAGTTGAGAATTCTAATTTGT	34.8	29.2	56.3	54.0	217 bp	1.0	45.2
ycf1_p2	ACCAATAACGATTCATACAAGCT	AGAGAGTTGAGAATTCTAATTTGTT	34.8	28.0	56.3	54.7	217 bp	1.0	45.2
ycf1_p3	ACCAATAACGATTCATACAAGCT	AGAGTTGAGAATTCTAATTTGTTTCA	34.8	26.9	56.3	55.6	215 bp	1.0	45.2
ycf1_p4	ACCAATAACGATTCATACAAGCT	GAGAGTTGAGAATTCTAATTTGTTTCA	34.8	29.6	56.3	56.6	216 bp	1.0	45.2
ycf1_p5	AACCAATAACGATTCATACAAGCT	AGAGAGTTGAGAATTCTAATTTGT	33.3	29.2	57.0	54.0	218 bp	1.0	45.1