Hotspot

ndhF

SSC · 110890-113157 · 2268 bp

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Pi 0.0231

MarkerSeek score 58.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2268
variable_sites: 77
pi: 0.0231
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGACACTCCAGCTCGATTTC	50.0	50.0	58.0	57.3	2942 bp	0.333333	47.7
ndhF_p2	ACAGGAACAAGAGGGATCCA	GACACTCCAGCTCGATTTCT	50.0	50.0	58.0	57.3	2941 bp	0.333333	47.7
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGACACTCCAGCTCGATTTC	50.0	50.0	58.0	57.3	2934 bp	0.333333	47.6
ndhF_p4	AAGAGGGATCCACCGAAGAA	GACACTCCAGCTCGATTTCT	50.0	50.0	58.0	57.3	2933 bp	0.333333	47.6
ndhF_p5	GCTTCCGAAATGAAGGGGAT	GACACTCCAGCTCGATTTCT	50.0	50.0	57.9	57.3	2964 bp	0.333333	47.6