Hotspot

ndhD-psaC

SSC · 117758-117850 · 93 bp

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Pi 0.058

MarkerSeek score 72.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 8
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TCTTTCCTCCCCACATGGAT	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	720-721 bp	0.666667	68.8
ndhD-psaC_p2	TCTTTCCTCCCCACATGGAT	AAGATGTGAATCCGCCTGTC	50.0	50.0	58.0	58.0	685-686 bp	0.666667	68.6
ndhD-psaC_p3	TCTTTCCTCCCCACATGGAT	CTGCTCCAAGAACAGAGGAC	50.0	55.0	58.0	57.9	719-720 bp	0.666667	68.2
ndhD-psaC_p4	TCTTTCCTCCCCACATGGAT	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	758-759 bp	0.666667	67.8
ndhD-psaC_p5	TCTTTCCTCCCCACATGGAT	TGCTTCTGCTCCAAGAACAG	50.0	50.0	58.0	58.1	725 bp	0.333333	52.3