Hotspot

rpl32-trnL-UAG

SSC · 114113-114829 · 717 bp

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Pi 0.049
MarkerSeek score 85.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
717
variable_sites
50
pi
0.049
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGTTTTGTTGTGCGACAAACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 57.7 57.9 786-801 bp 1.0 76.6
rpl32-trnL-UAG_p2 AGTTTTGTTGTGCGACAAACA AGCAGCGTGTCTACCAATTT 38.1 45.0 57.7 57.8 779-794 bp 1.0 76.2
rpl32-trnL-UAG_p3 AGTTTTGTTGTGCGACAAACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 57.7 57.8 785-800 bp 1.0 76.0
rpl32-trnL-UAG_p4 AGTTTTGTTGTGCGACAAACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 57.7 57.8 784-799 bp 1.0 76.0
rpl32-trnL-UAG_p5 AGTTTTGTTGTGCGACAAACA GAGCAGCGTGTCTACCAATT 38.1 50.0 57.7 58.3 780-795 bp 1.0 75.4