Hotspot

petD-rpoA

LSC · 78596-78789 · 194 bp

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Pi 0.0533

MarkerSeek score 75.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 15
pi: 0.0533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTTTGATCGGTACCGCAGTT	ACATTTCGCAGTTGATTTACCA	45.0	36.4	57.8	56.9	317-332 bp	1.0	57.4
petD-rpoA_p2	TTTTTGATCGGTACCGCAGT	ACATTTCGCAGTTGATTTACCA	45.0	36.4	57.8	56.9	318-333 bp	1.0	57.4
petD-rpoA_p3	TTGGGGATTGGTGCAACATT	ACATTTCGCAGTTGATTTACCA	45.0	36.4	58.3	56.9	288-303 bp	1.0	57.0
petD-rpoA_p4	TTTTGATCGGTACCGCAGTT	AACATTTCGCAGTTGATTTACCA	45.0	34.8	57.8	57.6	318-333 bp	1.0	54.7
petD-rpoA_p5	TTTTGATCGGTACCGCAGTT	ACATTTCGCAGTTGATTTACCAA	45.0	34.8	57.8	57.6	317-332 bp	1.0	54.7