Hotspot

ndhC-trnV-UAC

LSC · 50896-51943 · 1048 bp

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Pi 0.0382

MarkerSeek score 74.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 59
pi: 0.0382
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCTAGTCGGGGCTAAAACTC	GTTCGAGTCCGTATAGCCCT	55.0	55.0	58.1	58.7	1149-1222 bp	1.0	81.0
ndhC-trnV-UAC_p2	TTGCTAGTCGGGGCTAAAAC	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1151-1224 bp	1.0	80.7
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1142-1215 bp	1.0	80.4
ndhC-trnV-UAC_p4	GCTAGTCGGGGCTAAAACTC	TTCGAGTCCGTATAGCCCTA	55.0	50.0	58.1	57.1	1148-1221 bp	1.0	79.0
ndhC-trnV-UAC_p5	TTGCTAGTCGGGGCTAAAAC	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.9	57.1	1150-1223 bp	1.0	78.6