Hotspot

trnS-GGA-rps4

LSC · 45572-45847 · 276 bp

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Pi 0.0326

MarkerSeek score 73.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 276
variable_sites: 13
pi: 0.0326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	TTTGACCCTTCACCCATTCC	50.0	50.0	58.1	57.7	415-440 bp	1.0	82.3
trnS-GGA-rps4_p2	AGGCTTTTGTTTACCGAGGG	GACCCTTCACCCATTCCAAT	50.0	50.0	58.1	57.5	412-437 bp	1.0	80.5
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	TTTGACCCTTCACCCATTCC	50.0	50.0	58.4	57.7	414-439 bp	1.0	79.9
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	TTTGACCCTTCACCCATTCC	55.0	50.0	58.4	57.7	467-492 bp	1.0	79.4
trnS-GGA-rps4_p5	TTCACTCGGTTTTTGGGTCA	TTTGACCCTTCACCCATTCC	45.0	50.0	57.6	57.7	495-520 bp	1.0	78.8