Hotspot

psaA-ycf3

LSC · 42459-43133 · 675 bp

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Pi 0.0188

MarkerSeek score 72.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 18
pi: 0.0188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTGGCCCATTCTTCGAAAGA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	856-972 bp	1.0	81.3
psaA-ycf3_p2	TCTGGCCCATTCTTCGAAAG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	857-973 bp	1.0	81.3
psaA-ycf3_p3	CTGGCCCATTCTTCGAAAGA	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	838-954 bp	1.0	81.3
psaA-ycf3_p4	TCTGGCCCATTCTTCGAAAG	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	839-955 bp	1.0	81.3
psaA-ycf3_p5	GTCTGGCCCATTCTTCGAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.2	58.0	858-974 bp	1.0	80.7