Hotspot

psbM-trnD-GUC

LSC · 30293-31234 · 942 bp

Back to Heliotropium

Pi 0.0287

MarkerSeek score 71.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 40
pi: 0.0287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.4	58.5	1074-1267 bp	1.0	58.9
psbM-trnD-GUC_p2	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.4	58.5	1066-1259 bp	1.0	58.8
psbM-trnD-GUC_p3	AAGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1067-1260 bp	1.0	57.2
psbM-trnD-GUC_p4	AGCGGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1066-1259 bp	1.0	57.2
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.1	58.5	1075-1268 bp	1.0	56.7