Hotspot

trnC-GCA-petN

LSC · 28080-28866 · 787 bp

Back to Heliotropium

Pi 0.0246
MarkerSeek score 71.90
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
787
variable_sites
28
pi
0.0246
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 897-988 bp 1.0 84.9
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 895-986 bp 1.0 84.9
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 898-989 bp 1.0 84.3
trnC-GCA-petN_p4 GTATCATTTTGGCGGCATGG TTCTTCCCCACACTACGAGT 50.0 50.0 57.9 58.0 947-1038 bp 1.0 84.2
trnC-GCA-petN_p5 GTATCATTTTGGCGGCATGG ACTTCTTCCCCACACTACGA 50.0 50.0 57.9 58.0 949-1040 bp 1.0 84.1