Hotspot

rps16-trnQ-UUG

LSC · 6133-6767 · 635 bp

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Pi 0.0386

MarkerSeek score 74.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 36
pi: 0.0386
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCTTTCTGCCACATCGTTTC	GCTATTCGGAGGTTCGAGTC	50.0	55.0	58.0	57.9	704-1120 bp	1.0	83.0
rps16-trnQ-UUG_p2	GCTTTCTGCCACATCGTTTC	TATTCGGAGGTTCGAGTCCT	50.0	50.0	58.0	57.6	702-1118 bp	1.0	80.5
rps16-trnQ-UUG_p3	GCTTTCTGCCACATCGTTTC	ATTCGGAGGTTCGAGTCCTT	50.0	50.0	58.0	58.4	701-1117 bp	1.0	80.4
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GCTATTCGGAGGTTCGAGTC	55.0	55.0	58.4	57.9	731-1147 bp	1.0	80.1
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GCTATTCGGAGGTTCGAGTC	55.0	55.0	58.4	57.9	730-1146 bp	1.0	80.1