Hotspot

trnH-GUG-psbA

LSC · 93-397 · 305 bp

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Pi 0.0759

MarkerSeek score 75.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 30
pi: 0.0759
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GTTGATAGTCAAGGCCGTGT	50.0	50.0	57.9	57.9	531 bp	0.333333	52.0
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	GGCTGAGCACAACATCCTTA	50.0	50.0	57.9	57.9	889 bp	0.333333	51.8
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	GTATTCCAGGCTGAGCACAA	50.0	50.0	57.9	57.9	897 bp	0.333333	51.8
trnH-GUG-psbA_p4	ATATTATGGGCGAACGACGG	TATTCCAGGCTGAGCACAAC	50.0	50.0	57.9	57.9	896 bp	0.333333	51.8
trnH-GUG-psbA_p5	ATATTATGGGCGAACGACGG	TTGTATTCCAGGCTGAGCAC	50.0	50.0	57.9	57.9	899 bp	0.333333	51.8