Hotspot

ndhF-rpl32

SSC · 111240-112081 · 842 bp

Back to Heliophila

Pi 0.0474
MarkerSeek score 83.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
842
variable_sites
144
pi
0.0474
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCAATAGGTCGTGTGAACCA TGCATCAAGTTTGTCCTAGGG 50.0 47.6 58.2 57.9 1657-1724 bp 0.625 66.7
ndhF-rpl32_p2 TGCAATAGGTCGTGTGAACC TGCATCAAGTTTGTCCTAGGG 50.0 47.6 58.2 57.9 1658-1725 bp 0.625 66.7
ndhF-rpl32_p3 GGTCGTGTGAACCAAAAACC TGCATCAAGTTTGTCCTAGGG 50.0 47.6 57.8 57.9 1651-1718 bp 0.625 66.7
ndhF-rpl32_p4 GTCCACATACGTCGAAGGTT TGCATCAAGTTTGTCCTAGGG 50.0 47.6 57.6 57.9 1267-1334 bp 0.625 65.6
ndhF-rpl32_p5 AGTCCACATACGTCGAAGGT TGCATCAAGTTTGTCCTAGGG 50.0 47.6 58.5 57.9 1268-1335 bp 0.625 64.8