Hotspot

ndhC-trnV

LSC · 49315-50180 · 866 bp

Back to Heliophila

Pi 0.042

MarkerSeek score 86.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 142
pi: 0.042
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	58.1	58.7	795-1041 bp	1.0	77.5
ndhC-trnV_p2	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	42.9	50.0	58.1	57.1	794-1040 bp	1.0	75.5
ndhC-trnV_p3	TGCCAAAACAGGAATAGCAC	GTTCGAGTCCGTATAGCCCT	45.0	55.0	56.6	58.7	795-1041 bp	1.0	75.4
ndhC-trnV_p4	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCAG	42.9	47.6	58.1	58.1	823-1069 bp	1.0	74.5
ndhC-trnV_p5	TGCCAAAACAGGAATAGCACT	TCGAACTCTTTTACCGAGCA	42.9	45.0	58.1	56.9	823-1069 bp	1.0	74.3