Hotspot

trnF-ndhJ

LSC · 46943-47625 · 683 bp

Back to Heliophila

Pi 0.0377

MarkerSeek score 81.00

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 99
pi: 0.0377
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	663-857 bp	1.0	91.5
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	50.0	50.0	58.1	57.9	660-854 bp	1.0	90.9
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGATAGCCATCCACGACTGA	50.0	50.0	58.1	58.2	670-864 bp	1.0	89.8
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATCCACGACTGAAACGGATC	50.0	50.0	58.1	57.7	662-856 bp	1.0	89.2
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	794 bp	0.0625	39.4