Hotspot

trnE-trnT

LSC · 29584-30416 · 833 bp

Back to Heliophila

Pi 0.0333

MarkerSeek score 83.60

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 122
pi: 0.0333
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	913-939 bp	1.0	89.8
trnE-trnT_p2	ACTTCCTTTCGTGGTACCCT	GAACCGATGACTTACGCCTT	50.0	50.0	58.3	58.0	957-983 bp	1.0	89.8
trnE-trnT_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	892-918 bp	1.0	89.7
trnE-trnT_p4	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	892-918 bp	1.0	89.4
trnE-trnT_p5	ACTTCCTTTCGTGGTACCCT	CCATGGCGTTACTCTACCAC	50.0	55.0	58.3	58.1	936-962 bp	1.0	89.3