Hotspot

atpI-rps2

LSC · 13793-14037 · 245 bp

Back to Heliophila

Pi 0.0499

MarkerSeek score 89.20

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 245
variable_sites: 55
pi: 0.0499
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	TGGCCTACTTCTACACCAGA	TTCCAGCAAATGATGACGCT	50.0	45.0	57.4	58.2	420-446 bp	1.0	83.2
atpI-rps2_p2	TGGCCTACTTCTACACCAGA	GCAATTTGTGAGGGTCGTTC	50.0	50.0	57.4	57.7	361-387 bp	1.0	82.2
atpI-rps2_p3	TGGCCTACTTCTACACCAGA	TTGTGAGGGTCGTTCTAGCT	50.0	50.0	57.4	58.4	356-382 bp	1.0	81.9
atpI-rps2_p4	TGGCCTACTTCTACACCAGA	TTTGTGAGGGTCGTTCTAGC	50.0	50.0	57.4	57.5	357-383 bp	1.0	81.2
atpI-rps2_p5	TGGCCTACTTCTACACCAGA	TGTGAGGGTCGTTCTAGCTA	50.0	50.0	57.4	57.5	355-381 bp	1.0	80.8