Hotspot

trnR-atpA

LSC · 8638-8942 · 305 bp

Back to Heliophila

Pi 0.0745

MarkerSeek score 77.80

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 73
pi: 0.0745
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	TGATCACTCGACGTCGACTA	GGGTCGTGAAGCTTATCCAG	50.0	55.0	58.0	58.1	1250-1313 bp	0.9375	89.0
trnR-atpA_p2	TGATCACTCGACGTCGACTA	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1249-1312 bp	0.9375	89.0
trnR-atpA_p3	AGCCTTCCAAGCTAACGATG	GGGTCGTGAAGCTTATCCAG	50.0	55.0	58.0	58.1	1222-1285 bp	0.9375	88.8
trnR-atpA_p4	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1221-1284 bp	0.9375	88.8
trnR-atpA_p5	ACCCTTAGCCTTCCAAGCTA	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1227-1290 bp	0.9375	88.8