Hotspot

trnG-trnR

LSC · 8424-8565 · 142 bp

Back to Heliophila

Pi 0.0852

MarkerSeek score 83.80

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 43
pi: 0.0852
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	TGATCACTCGACGTCGACTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	278-316 bp	0.875	54.4
trnG-trnR_p2	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	250-288 bp	0.875	54.2
trnG-trnR_p3	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	256-294 bp	0.875	54.1
trnG-trnR_p4	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	270-308 bp	0.875	53.9
trnG-trnR_p5	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	258-296 bp	0.875	53.8