Hotspot

trnS-trnG

LSC · 6944-7631 · 688 bp

Back to Heliophila

Pi 0.0473

MarkerSeek score 84.90

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 128
pi: 0.0473
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	732-816 bp	1.0	62.4
trnS-trnG_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	730-814 bp	1.0	62.1
trnS-trnG_p3	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	50.0	36.4	57.8	56.2	733-817 bp	1.0	60.5
trnS-trnG_p4	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	727-811 bp	1.0	60.4
trnS-trnG_p5	AACGCTTTAGTCCACTCAGC	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	731-815 bp	1.0	60.2