Hotspot

trnK-rps16

LSC · 4332-5042 · 711 bp

Back to Heliophila

Pi 0.0559

MarkerSeek score 83.20

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 154
pi: 0.0559
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CCTTTCAGGATCAGTCGTGG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	1472-2349 bp	1.0	91.3
trnK-rps16_p2	TCAGGATCAGTCGTGGTCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1468-2345 bp	0.9375	88.3
trnK-rps16_p3	TTCAGGATCAGTCGTGGTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1469-2346 bp	0.9375	88.3
trnK-rps16_p4	CCTTTCAGGATCAGTCGTGG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	58.0	57.9	947-1053 bp	0.75	80.8
trnK-rps16_p5	CCTTTCAGGATCAGTCGTGG	GATTTCCTTAAAACGGGCGC	55.0	50.0	58.0	58.1	945-962 bp	0.25	52.4