Hotspot

clpP1

LSC · 77803-78540 · 738 bp

Back to Helicteres

Pi 0.0325

MarkerSeek score 62.70

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 738
variable_sites: 24
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TACGACGTAACCGACTACGA	TATGCACACGATACCAAGGC	50.0	50.0	58.0	58.1	1641-1947 bp	1.0	84.5
clpP1_p2	CGTTTACGACGTAACCGACT	TATGCACACGATACCAAGGC	50.0	50.0	58.0	58.1	1645-1951 bp	1.0	84.2
clpP1_p3	TACGACGTAACCGACTACGA	GGAGGGATCAAAAACAGCCA	50.0	50.0	58.0	58.1	1757-2063 bp	1.0	84.0
clpP1_p4	TACGACGTAACCGACTACGA	GAACATGCCTTGTCTCCACA	50.0	50.0	58.0	58.1	1796-2102 bp	1.0	83.7
clpP1_p5	GGAAAGAAGACGAAGCGACT	TATGCACACGATACCAAGGC	50.0	50.0	57.9	58.1	1321 bp	0.5	59.0