Hotspot

accD

LSC · 63684-65636 · 1953 bp

Back to Helicteres

Pi 0.0328

MarkerSeek score 61.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1953
variable_sites: 64
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCAGGATTCTCCCAAACGAG	GCGGGCTTTGACTTTGATTC	55.0	50.0	58.0	58.0	2294-2458 bp	1.0	82.6
accD_p2	CTCCAGGATTCTCCCAAACG	GCGGGCTTTGACTTTGATTC	55.0	50.0	58.0	58.0	2296-2460 bp	1.0	82.5
accD_p3	CCAGGATTCTCCCAAACGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2880 bp	0.5	59.5
accD_p4	CTCCAGGATTCTCCCAAACG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2882 bp	0.5	59.5
accD_p5	CCAGGATTCTCCCAAACGAG	GAGGGAAAGCTGCTGAAAGT	55.0	50.0	58.0	58.1	2833 bp	0.5	59.0