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clpP1-psbB

LSC · 78541-79057 · 517 bp

Back to Helicteres

Pi 0.134

MarkerSeek score 79.90

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 68
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	TCACGAAAATGACTAGCGGG	CAGATAGCTGCCAAGAAGCA	50.0	50.0	58.0	58.0	1256-1347 bp	1.0	85.2
clpP1-psbB_p2	TCACGAAAATGACTAGCGGG	AGGTTGTACCCTTCCGGTTA	50.0	50.0	58.0	58.0	1458-1549 bp	1.0	84.6
clpP1-psbB_p3	TCACGAAAATGACTAGCGGG	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1423 bp	0.5	59.8
clpP1-psbB_p4	GTTGTCGGTAAAGCACGTTG	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1113 bp	0.5	59.7
clpP1-psbB_p5	GCCATTGAAGCAACCAATCC	ACACCCATATTCCAGGACCA	50.0	50.0	58.0	58.0	1330 bp	0.5	59.7