Hotspot

rps16-trnQ-UUG

LSC · 6760-7452 · 693 bp

Back to Helicteres

Pi 0.00752

MarkerSeek score 66.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 693
variable_sites: 5
pi: 0.00752
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AGTGGTGGGTTTTGACATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	784-805 bp	1.0	82.7
rps16-trnQ-UUG_p2	TGCTTTCTACCACAGCGTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	734-755 bp	1.0	82.6
rps16-trnQ-UUG_p3	TTGCTTTCTACCACAGCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	735-756 bp	1.0	82.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	745-766 bp	1.0	81.8
rps16-trnQ-UUG_p5	TTTTGACATCCACAGCCGAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	775-796 bp	1.0	81.2