Hotspot

trnN-GUU-trnR-ACG

IRa · 136598-137170 · 573 bp

Back to Heliconia

Pi 0.00908

MarkerSeek score 65.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 573
variable_sites: 10
pi: 0.00908
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	709-714 bp	1.0	79.9
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	746-751 bp	1.0	79.3
trnN-GUU-trnR-ACG_p3	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	673-678 bp	1.0	78.8
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	660-665 bp	1.0	78.8
trnN-GUU-trnR-ACG_p5	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	661-666 bp	1.0	78.4