Hotspot

trnL-UAG-ccsA

SSC · 120578-120666 · 89 bp

Back to Heliconia

Pi 0.0337

MarkerSeek score 64.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 89
variable_sites: 6
pi: 0.0337
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ATCACGCAATCCCACGATTT	50.0	45.0	57.9	58.2	267-279 bp	1.0	78.8
trnL-UAG-ccsA_p2	TAGACACGCTGCTCTTAGGA	AATCACGCAATCCCACGATT	50.0	45.0	57.9	58.2	268-280 bp	1.0	78.7
trnL-UAG-ccsA_p3	TAAGCCGCCATGGTGAAATT	AATCACGCAATCCCACGATT	45.0	45.0	58.2	58.2	290-302 bp	1.0	78.4
trnL-UAG-ccsA_p4	TAAGCCGCCATGGTGAAATT	ATCACGCAATCCCACGATTT	45.0	45.0	58.2	58.2	289-301 bp	1.0	78.4
trnL-UAG-ccsA_p5	AAATTGGTAGACACGCTGCT	ATCACGCAATCCCACGATTT	45.0	45.0	57.8	58.2	274-286 bp	1.0	78.2