Hotspot

trnR-ACG-trnN-GUU

IRb · 114350-114922 · 573 bp

Back to Heliconia

Pi 0.00908

MarkerSeek score 65.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 573
variable_sites: 10
pi: 0.00908
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	709-714 bp	1.0	79.9
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	746-751 bp	1.0	79.3
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	673-678 bp	1.0	78.8
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	660-665 bp	1.0	78.8
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	661-666 bp	1.0	78.4