Hotspot

psaJ-rpl33

LSC · 72445-73015 · 571 bp

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Pi 0.00788
MarkerSeek score 63.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
571
variable_sites
9
pi
0.00788
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATCGTTTATTCCCGGATGCC ACATCATTTCGGGCACAACT 50.0 45.0 58.1 58.1 658-680 bp 1.0 82.6
psaJ-rpl33_p2 ATCGTTTATTCCCGGATGCC GGCACAACTGGTACATTCCA 50.0 50.0 58.1 58.1 647-669 bp 1.0 82.6
psaJ-rpl33_p3 ATCGTTTATTCCCGGATGCC TGACATCATTTCGGGCACAA 50.0 45.0 58.1 58.1 660-682 bp 1.0 82.5
psaJ-rpl33_p4 ATCGTTTATTCCCGGATGCC TTGACATCATTTCGGGCACA 50.0 45.0 58.1 58.1 661-683 bp 1.0 82.5
psaJ-rpl33_p5 GATGCCTTGTCATTCCCCTT ACATCATTTCGGGCACAACT 50.0 45.0 57.9 58.1 644-666 bp 1.0 82.3