Hotspot

petA-psbJ

LSC · 67933-68940 · 1008 bp

Back to Heliconia

Pi 0.00813

MarkerSeek score 55.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 20
pi: 0.00813
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.8	58.0	1134-1155 bp	1.0	80.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.8	58.0	1132-1153 bp	1.0	80.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GCTGGTGGGTACTGTAACTG	50.0	55.0	57.8	57.9	1201-1222 bp	1.0	79.6
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TGGTGGGTACTGTAACTGGT	50.0	50.0	57.8	57.9	1199-1220 bp	1.0	79.5
petA-psbJ_p5	GGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.3	58.0	1139-1160 bp	1.0	79.0