Hotspot

trnT-GGU-psbD

LSC · 35831-36933 · 1103 bp

Back to Heliconia

Pi 0.0102
MarkerSeek score 52.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1103
variable_sites
24
pi
0.0102
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAGGAAAGAGCAATAGGCCG 55.0 50.0 58.1 58.0 1282-1300 bp 1.0 81.1
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG CACCGTCACCATCTTCGAAT 55.0 50.0 58.1 58.0 1849-1867 bp 1.0 80.8
trnT-GGU-psbD_p3 GGGGTCGGTCTTGAAACAAT CACCGTCACCATCTTCGAAT 50.0 50.0 58.1 58.0 2084-2104 bp 1.0 80.8
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA CACCGTCACCATCTTCGAAT 50.0 50.0 58.1 58.0 1852-1870 bp 1.0 80.6
trnT-GGU-psbD_p5 AACGGGCCCTTTTAACTCAG CACCGTCACCATCTTCGAAT 50.0 50.0 58.1 58.0 1868-1886 bp 1.0 80.6