Hotspot

atpH-atpI

LSC · 15785-17013 · 1229 bp

Back to Heliconia

Pi 0.00765

MarkerSeek score 65.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 21
pi: 0.00765
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.0	58.6	1318-1373 bp	1.0	76.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	1290-1345 bp	1.0	76.8
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.9	57.4	1346-1401 bp	1.0	76.2
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	50.0	45.0	57.9	58.6	1374-1429 bp	1.0	76.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACGTTAGCCGC	45.0	45.0	58.2	58.6	1319-1374 bp	1.0	75.6