Hotspot

rps16-trnQ-UUG

LSC · 6131-7845 · 1715 bp

Back to Heliconia

Pi 0.00817
MarkerSeek score 59.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1715
variable_sites
30
pi
0.00817
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1799-1806 bp 1.0 80.9
rps16-trnQ-UUG_p2 CAGCCGATCATGTCCTTCAA GTGCCAACCGGTAAAAATGG 50.0 50.0 58.0 57.9 1864-1871 bp 1.0 80.8
rps16-trnQ-UUG_p3 GCCGATCATGTCCTTCAAGT GTGCCAACCGGTAAAAATGG 50.0 50.0 58.0 57.9 1862-1869 bp 1.0 80.8
rps16-trnQ-UUG_p4 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1797-1804 bp 1.0 80.8
rps16-trnQ-UUG_p5 AATCCACAGCCGATCATGTC GTGCCAACCGGTAAAAATGG 50.0 50.0 58.0 57.9 1870-1877 bp 1.0 80.7