Hotspot

rbcL

LSC · 56068-57495 · 1428 bp

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Pi 0

MarkerSeek score 29.40

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AACGCCTAATCCATGTCGAG	TGGGCCAAGTTTAATTGCAA	50.0	40.0	57.8	56.4	1551 bp	1.0	65.4
rbcL_p2	AACGCCTAATCCATGTCGAG	TTGGGCCAAGTTTAATTGCA	50.0	40.0	57.8	56.4	1552 bp	1.0	65.4
rbcL_p3	AACGCCTAATCCATGTCGAG	TTGGGCCAAGTTTAATTGCAA	50.0	38.1	57.8	57.2	1552 bp	1.0	63.4
rbcL_p4	AACGCCTAATCCATGTCGAG	GATTGGGCCAAGTTTAATTGCA	50.0	40.9	57.8	58.1	1554 bp	1.0	61.7
rbcL_p5	AACGCCTAATCCATGTCGAG	TGGGCCAAGTTTAATTGCAAT	50.0	38.1	57.8	56.9	1551 bp	1.0	61.7