Hotspot

psbA

LSC · 266-1327 · 1062 bp

Back to Helicia

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCGGTATTGCTACTTCAACA	AATTCGTGTGCTTGGGAGTC	42.9	50.0	58.0	58.5	1158 bp	1.0	67.9
psbA_p2	TGCGGTATTGCTACTTCAACA	TAATTCGTGTGCTTGGGAGT	42.9	45.0	58.0	57.1	1159 bp	1.0	64.9
psbA_p3	TGCGGTATTGCTACTTCAACA	GCTTGGGAGTCCCTGATGAT	42.9	55.0	58.0	59.2	1149 bp	1.0	62.4
psbA_p4	TGAAGGAGCAATACCAACACT	AATTCGTGTGCTTGGGAGTC	42.9	50.0	57.2	58.5	1196 bp	1.0	62.0
psbA_p5	TGCGGTATTGCTACTTCAACA	ATTCGTGTGCTTGGGAGTC	42.9	52.6	58.0	57.8	1157 bp	1.0	61.8