Hotspot

ndhF

SSC · 112213-114408 · 2196 bp

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Pi 0.000911

MarkerSeek score 57.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2196
variable_sites: 2
pi: 0.000911
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGACCTTATCGTGGAACA	GACTTGGTCATCTAAGCCGG	50.0	55.0	58.1	58.1	2331 bp	1.0	79.2
ndhF_p2	TGAAGGGACCTTATCGTGGA	GACTTGGTCATCTAAGCCGG	50.0	55.0	57.8	58.1	2334 bp	1.0	77.7
ndhF_p3	AGGGACCTTATCGTGGAACA	AGCGACTTGGTCATCTAAGC	50.0	50.0	58.1	57.7	2334 bp	1.0	77.2
ndhF_p4	AGGGACCTTATCGTGGAACA	CGACTTGGTCATCTAAGCCG	50.0	55.0	58.1	58.4	2332 bp	1.0	76.1
ndhF_p5	TGAAGGGACCTTATCGTGGA	AGCGACTTGGTCATCTAAGC	50.0	50.0	57.8	57.7	2337 bp	1.0	75.7