Hotspot

atpF

LSC · 11526-12878 · 1353 bp

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Pi 0.000739
MarkerSeek score 59.20
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1353
variable_sites
1
pi
0.000739
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 TTTCATCGGCTCGAATGGTT AAAAGGGGCGAAGTGATACA 45.0 45.0 57.9 57.1 1504 bp 1.0 72.2
atpF_p2 TTTCATCGGCTCGAATGGTT AAAGGGGCGAAGTGATACAA 45.0 45.0 57.9 57.1 1503 bp 1.0 72.2
atpF_p3 TTTCATCGGCTCGAATGGTT AAGGGGCGAAGTGATACAAA 45.0 45.0 57.9 57.1 1502 bp 1.0 72.2
atpF_p4 TTTCATCGGCTCGAATGGTT AGGGGCGAAGTGATACAAAA 45.0 45.0 57.9 57.1 1501 bp 1.0 72.2
atpF_p5 TCATCGGCTCGAATGGTTAC AGGGGCGAAGTGATACAAAA 50.0 45.0 57.8 57.1 1499 bp 1.0 71.3