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psbE-petL

LSC · 65913-66719 · 807 bp

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Pi 0.00124

MarkerSeek score 59.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTATAGTTGAAGCCGCCA	50.0	50.0	57.9	58.0	965 bp	1.0	79.4
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GGTTATAGTTGAAGCCGCCA	45.0	50.0	58.2	58.0	974 bp	1.0	78.5
psbE-petL_p3	TGCTGACGAATAACCAACCC	GGTTATAGTTGAAGCCGCCA	50.0	50.0	58.2	58.0	979 bp	1.0	78.3
psbE-petL_p4	CGAATAACCAACCCGCAATG	GGTTATAGTTGAAGCCGCCA	50.0	50.0	57.8	58.0	973 bp	1.0	78.3
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GGTTATAGTTGAAGCCGCCA	50.0	50.0	58.2	58.0	967 bp	1.0	77.9