Hotspot

ndhC-tRNA-Lys(TTT)

LSC · 51716-52235 · 520 bp

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Pi 0

MarkerSeek score 58.40

Primer available yes

Label ndhC-tRNA-Lys(TTT)

Feature Metadata

label_name: ndhC-tRNA-Lys(TTT)
feature_type: igs
region: LSC
length_bp: 520
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Lys(TTT)_p1	CTCTGGTCCTTCGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	663-670 bp	1.0	78.2
ndhC-tRNA-Lys(TTT)_p2	CTCTGGTCCTTCGCTAATCG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	57.9	57.5	662-669 bp	1.0	75.5
ndhC-tRNA-Lys(TTT)_p3	GCTAATCGGGGCCAAAATTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	651-658 bp	1.0	75.2
ndhC-tRNA-Lys(TTT)_p4	TAATCGGGGCCAAAATTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.6	57.8	649-656 bp	1.0	74.4
ndhC-tRNA-Lys(TTT)_p5	CTCTGGTCCTTCGCTAATCG	GTTCGAGTCCGTATAGCCCT	55.0	55.0	57.9	58.7	651-658 bp	1.0	74.4