Hotspot

tRNA-Glu(TTC)-tRNA-Thr(GGT)

LSC · 31531-32602 · 1072 bp

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Pi 0.000934

MarkerSeek score 59.40

Primer available yes

Label tRNA-Glu(TTC)-tRNA-Thr(GGT)

Feature Metadata

label_name: tRNA-Glu(TTC)-tRNA-Thr(GGT)
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 1
pi: 0.000934
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu(TTC)-tRNA-Thr(GGT)_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1212-1213 bp	1.0	78.8
tRNA-Glu(TTC)-tRNA-Thr(GGT)_p2	TTTCGTAGTACCCTACCCCC	GTGGAAAGAGCCCCTTATCG	55.0	55.0	57.8	58.0	1237-1238 bp	1.0	78.5
tRNA-Glu(TTC)-tRNA-Thr(GGT)_p3	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	1177-1178 bp	1.0	78.0
tRNA-Glu(TTC)-tRNA-Thr(GGT)_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	1174-1175 bp	1.0	78.0
tRNA-Glu(TTC)-tRNA-Thr(GGT)_p5	GCTGCCTCCTTGAAAGAGAG	GTGGAAAGAGCCCCTTATCG	55.0	55.0	58.3	58.0	1199-1200 bp	1.0	77.7