Hotspot

tRNA-Lys(TTT)-rps16

LSC · 4178-5209 · 1032 bp

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Pi 0.00194
MarkerSeek score 60.00
Primer available yes
Label tRNA-Lys(TTT)-rps16

Feature Metadata

label_name
tRNA-Lys(TTT)-rps16
feature_type
igs
region
LSC
length_bp
1032
variable_sites
2
pi
0.00194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys(TTT)-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1206-1207 bp 1.0 78.3
tRNA-Lys(TTT)-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1207-1208 bp 1.0 78.3
tRNA-Lys(TTT)-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1211-1212 bp 1.0 78.3
tRNA-Lys(TTT)-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1209-1210 bp 1.0 78.3
tRNA-Lys(TTT)-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1157-1158 bp 1.0 77.3